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Product Info Summary
| SKU: | M00699 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7]
View all Protein Kinase A regulatory subunit I alpha Antibodies
SKU/Catalog Number
M00699
Size
100 µl
Description
Boster Bio Anti-PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody, clone OTI6C7 (formerly 6C7). Catalog# M00699. Tested in IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00699)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI6C7
Isotype
IgG1
Immunogen
Full-length protein expressed in 293T cell transfected with human PRKAR1A expression vector
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00699 is reactive to PRKAR1A in Human, Mouse, Rat
Applications
M00699 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.982kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR1A monoclonal antibody.
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PRKAR1A monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Figure from citation: PRKAR1A IHC in a CNC-associated cardiac myxoma. A
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Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PRKAR1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PRKAR1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-PRKAR1A mouse monoclonal antibody (M00699) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1A.
Protein Target Info & Infographic
Gene/Protein Information For PRKAR1A (Source: Uniprot.org, NCBI)
Gene Name
PRKAR1A
Full Name
cAMP-dependent protein kinase type I-alpha regulatory subunit
Weight
42.982kDa
Superfamily
cAMP-dependent kinase regulatory chain family
Alternative Names
cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; CNC; CNC1; MGC17251; PKA R1 alpha; PKA1; PKR1; PPNAD1; PRKAR1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1); Tissue-specific extinguisher 1; TSE1DKFZp779L0468 PRKAR1A ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, TSE1 protein kinase cAMP-dependent type I regulatory subunit alpha cAMP-dependent protein kinase type I-alpha regulatory subunit|Carney complex type 1|cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|epididymis secretory sperm binding protein|protein kinase A type 1a regulatory subunit|protein kinase, cAMP-dependent, regulatory subunit type I alpha|protein kinase, cAMP-dependent, regulatory, type I, alpha|tissue-specific extinguisher 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKAR1A, check out the PRKAR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKAR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein Kinase A regulatory subunit I alpha (PRKAR1A) Mouse Monoclonal Antibody [Clone ID: OTI6C7] (M00699)
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